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Nucleic acid purification from plants, animals and

Nucleic acid purification from plants, animals and

Nucleic acid purification from plants, animals and ...

Nucleic acid purification from plants, animals and microbes in under 30 seconds PLoS Biol. 2017 Nov 21;15(11): e2003916. ... an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed ...

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Nucleic acid purification from plants, animals and ...

21/11/2017  For nucleic acid purification from plant tissues, 5–10 mg of leaf tissue was ground in a 1.5-ml tube with a plastic pestle in presence of 50 μl extraction buffer #1 (50 mM Tris [pH 8.0], 150 mM NaCl, 2% PVP, 1% Tween-20) for approximately 30 seconds. A 3-mm diameter disc was cut from a piece of Whatman No.1 using a hole puncher and transferred into the tissue extract for a minimum of three ...

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Correction: Nucleic acid purification from plants, animals ...

07/05/2018  Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds. Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, Trau M, Botella JR. Erratum for PLoS Biol. 2017 Nov 21;15(11):e2003916.

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Nucleic acid purification from plants, animals and ...

21/11/2017  Nucleic acid purification from plants, animals and microbes in under 30 seconds. Coronavirus: ... For nucleic acid purification from plant tissues, 5–10 mg of leaf tissue was ground in a 1.5-ml tube with a plastic pestle in presence of 50 μl extraction buffer #1 (50 mM Tris [pH 8.0], 150 mM NaCl, 2% PVP, 1% Tween-20) for approximately 30 seconds. A 3-mm diameter disc was cut from a

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Nucleic acid purification from plants, animals and ...

Nucleic acid purification from plants, animals and microbes in under 30 seconds Yiping Zou1‡, Michael Glenn Mason1‡*, Yuling Wang2, Eugene Wee2, Conny Turni3, Patrick J. Blackall3, Matt Trau2, Jose Ramon Botella1* 1 Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, The University of Queensland, St.Lucia, Australia, 2 Centre for Personalized Nanomedicine ...

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Correction: Nucleic acid purification from plants, animals ...

07/05/2018  Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds. Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, Trau M, Botella JR. Erratum for PLoS Biol. 2017 Nov 21;15(11):e2003916.

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Correction: Nucleic acid purification from plants, animals ...

Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds PLoS Biol . 2018 May 7;16(5):e1002630. doi: 10.1371/journal.pbio.1002630.

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(PDF) Nucleic acid purification from plants, animals and ...

21/11/2017  For nucleic acid purification from plant tissues, 5–10 mg of leaf tissue was ground in a 1.5-ml tube with a plastic pestle in presence of 50 μ l extraction buffer #1 (50 mM Tris [pH 8.0], 150 ...

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Nucleic acid purification from plants, animals and ...

Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed of this method as well as the low cost and availability of suitable ...

get price

[PDF] Nucleic acid purification from plants, animals and ...

Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed of this method as well as the low cost and availability of suitable ...

get price

Nucleic acid purification from plants, animals and ...

Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification.

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Correction: Nucleic acid purification from plants, animals ...

07/05/2018  Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals

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Correction: Nucleic acid purification from plants, animals ...

07/05/2018  This corrects the article "Nucleic acid purification from plants, animals and microbes in under 30 seconds" in volume 15, e2003916. This article has been cited by other articles in PMC. The Competing interests statement for this paper is incorrect.

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Nucleic acid purification from plants, animals and ...

22/11/2017  Nucleic acid purification from plants, animals and microbes in under 30 seconds. Yiping Zou et al (2017), PLOS Biology https://doi/10.1371/journal.pbio.2...

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Rapid (30-second), equipment-free purification of nucleic ...

01/10/2020  Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick. Nucleic acids can be purified by dipping in ...

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[PDF] Nucleic acid purification from plants, animals and ...

Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed of this method as well as the low cost and availability of suitable ...

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Nucleic acid purification from plants, animals and ...

A novel plant DNA extraction method using filter paper-based 96-well spin plate (2017) Rui Shi et al. PLANTA A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples

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Nucleic acid purification from plants, animals and ...

As a control, 1 l of the raw lysate was added directly into a separate PCR - "Nucleic acid purification from plants, animals and microbes in under 30 seconds" Fig 3. DNA and RNA extraction from plant and animal pathogens using cellulose-based paper. (A) DNA was purified from P. syringae-infected Arabidopsis leaves at different stages of infection using cellulose discs and a fragment of the P ...

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Correction: Nucleic acid purification from plants, animals ...

07/05/2018  Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals

get price

Nucleic acid purification from plants, animals and ...

Nucleic acid purification from plants, animals and microbes in under 30 seconds PLOS Biology ... we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and ...

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Nucleic acid purification from plants, animals and ...

Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biol. 2017; 15(11):e2003916 (ISSN: 1545-7885) Zou Y; Mason MG; Wang Y; Wee E; Turni C; Blackall PJ; Trau M; Botella JR . Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to ...

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Rapid (30-second), equipment-free purification of nucleic ...

01/10/2020  Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick. Nucleic acids can be purified by dipping in ...

get price

Nucleic Acid Purification – magtivio

Nucleic Acid Purification. From pathogens, vegetal and animal tissues, cells and body fluids. MagSi-NA Pathogens. Allows for the cost-effective extraction of DNA and RNA from a variety of sample materials like serum, plasma, oropharyngeal or nasopharyngeal swab, or any other respiratory samples. Purified total nucleic acids can be used for qPCR based or any other enzymatic pathogen detection ...

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MODULE 4- LECTURE 1 ISOLATION AND PURIFICATION OF NUCLEIC

Purification of nucleic acids . 4-1.2. Isolation and Purification of Genomic DNA. Genomic DNA is found in the nucleus of all living cells with the structure of double- stranded DNA remaining unchanged (helical ribbon). The isolation of genomic DNA differs in animals and plant cells. DNA isolation from plant cells is difficult due to the presence of cell wall, as compared to animal cells. The ...

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Nucleic acid extraction and purification technologies

Protocols have been optimised for both plant and animal purifications, and the Kleargene chemistry can generate yields between 100 ng and 10 µg DNA from samples ranging from plant leaf tissue and plant seeds to rodent tails (Figure 4). Kleargene has been highly optimised for automation with both 96-well formats (plant and tissue) and 384-well formats (plant), achieving higher throughput than ...

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